NdoA

• Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
  
  

Gene name	ndoA
Synonyms	ydcE, mazF
Essential	no
Product	mRNA interferase
Function	unknown
Gene expression levels in SubtiExpress: ndoA
Interactions involving this protein in SubtInteract: NdoA
MW, pI	12 kDa, 4.892
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ndoAI, rsbR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU04660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed

• Protein family: pemK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity: the activity is inhibited by the interaction with NdoAI PubMed

• Interactions:
  • NdoA(2)-NdoAI(2)-NdoA(2) PubMed
  • the active protein forms dimers PubMed

• Localization:

Database entries

• Structure:
  • 1NE8 PubMed
  • 4MDX (NdoA-RNA) PubMed
  • 4ME7 (NdoA-NdoAI complex) PubMed

• UniProt: P96622

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ndoAI-ndoA PubMed

• Expression browser: ndoA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for ndoA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: