PolC

• Description: DNA polymerase III (alpha subunit), part of the replisome
  
  

Gene name	polC
Synonyms	mutI, dnaF, dnaP
Essential	yes PubMed
Product	DNA polymerase III (alpha subunit)
Function	DNA replication
Gene expression levels in SubtiExpress: polC
Interactions involving this protein in SubtInteract: PolC
MW, pI	162 kDa, 5.135
Gene length, protein length	4311 bp, 1437 aa
Immediate neighbours	proS, ylxS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU16580

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication PubMed

• Protein family: PolC subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-PolC PubMed

• Localization:
  • forms foci PubMed
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:
  • 3F2B (from Geobacillus kaustophilus, 71% identity) PubMed

• UniProt: P13267

• KEGG entry: [2]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon:

• Expression browser: polC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Kestutis Timinskas, Monika Balvočiūtė, Albertas Timinskas, Česlovas Venclovas
Comprehensive analysis of DNA polymerase III α subunits and their homologs in bacterial genomes.
Nucleic Acids Res: 2014, 42(3);1393-413
[PubMed:24106089] [WorldCat.org] [DOI] (I p)

Motohiro Akashi, Hirofumi Yoshikawa
Relevance of GC content to the conservation of DNA polymerase III/mismatch repair system in Gram-positive bacteria.
Front Microbiol: 2013, 4;266
[PubMed:24062730] [WorldCat.org] [DOI] (P e)

Olivier Rannou, Emmanuelle Le Chatelier, Marilynn A Larson, Hamid Nouri, Bérengère Dalmais, Charles Laughton, Laurent Jannière, Panos Soultanas
Functional interplay of DnaE polymerase, DnaG primase and DnaC helicase within a ternary complex, and primase to polymerase hand-off during lagging strand DNA replication in Bacillus subtilis.
Nucleic Acids Res: 2013, 41(10);5303-20
[PubMed:23563155] [WorldCat.org] [DOI] (I p)

Elena M Seco, John C Zinder, Carol M Manhart, Ambra Lo Piano, Charles S McHenry, Silvia Ayora
Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro.
Nucleic Acids Res: 2013, 41(3);1711-21
[PubMed:23268446] [WorldCat.org] [DOI] (I p)

Andrew D Klocko, Jeremy W Schroeder, Brian W Walsh, Justin S Lenhart, Margery L Evans, Lyle A Simmons
Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork.
Mol Microbiol: 2011, 82(3);648-63
[PubMed:21958350] [WorldCat.org] [DOI] (I p)

Kęstutis Timinskas, Česlovas Venclovas
The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III τ subunit.
FEBS J: 2011, 278(17);3109-18
[PubMed:21740522] [WorldCat.org] [DOI] (I p)

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Ronald J Evans, Douglas R Davies, James M Bullard, Jeffrey Christensen, Louis S Green, Joseph W Guiles, Janice D Pata, Wendy K Ribble, Nebojsa Janjic, Thale C Jarvis
Structure of PolC reveals unique DNA binding and fidelity determinants.
Proc Natl Acad Sci U S A: 2008, 105(52);20695-700
[PubMed:19106298] [WorldCat.org] [DOI] (I p)

Megan E Rokop, Jennifer M Auchtung, Alan D Grossman
Control of DNA replication initiation by recruitment of an essential initiation protein to the membrane of Bacillus subtilis.
Mol Microbiol: 2004, 52(6);1757-67
[PubMed:15186423] [WorldCat.org] [DOI] (P p)

E Dervyn, C Suski, R Daniel, C Bruand, J Chapuis, J Errington, L Jannière, S D Ehrlich
Two essential DNA polymerases at the bacterial replication fork.
Science: 2001, 294(5547);1716-9
[PubMed:11721055] [WorldCat.org] [DOI] (P p)