YabT

• Description: Ser/Thr kinase, controls DNA integrity during spore development
  
  

Gene name	yabT
Synonyms
Essential	no
Product	Ser/Thr kinase
Function	control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI	37 kDa, 9.943
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yabS, tilS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU00660

Phenotypes of a mutant

• increased sensitivity to DNA damage during spore development PubMed

Database entries

• BsubCyc: BSU00660

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylation of RecA on Ser-2 PubMed

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • autophosphorylation is stimulated by non-specific binding to DNA PubMed

• Interactions:
  • YabT-RecA PubMed

• Localization:
  • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

• BsubCyc: BSU00660

• Structure:

• UniProt: P37562

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yabT PubMed

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP577 (erm), available in Jörg Stülke's lab

• Expression vector:
  • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab

• lacZ fusion:
• translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody: