DefB

• Description: N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine
  
  

Gene name	defB
Synonyms	ykrB
Essential	no
Product	N-formylcysteine deformylase
Function	utilization of S-methyl-cysteine
Gene expression levels in SubtiExpress: defB
MW, pI	20 kDa, 5.456
Gene length, protein length	552 bp, 184 aa
Immediate neighbours	ykrA, ykzV
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

The gene

Basic information

• Locus tag: BSU14560

Phenotypes of a mutant

• no growth with S-methyl cysteine PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-acetylcysteine --> cysteine + acetate PubMed

• Protein family: polypeptide deformylase family (according to Swiss-Prot)

• Paralogous protein(s): DefA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)

• UniProt: Q45495

• KEGG entry: [2]

• E.C. number: 3.5.1.88

Expression and regulation

• Operon:

• Expression browser: defB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 580 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 735 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997] [WorldCat.org] [DOI] (I p)

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)