Difference between revisions of "DgcW"
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|style="background:#ABCDEF;" align="center"| '''Product''' || diguanylate cyclase and potential phosphodiesterase | |style="background:#ABCDEF;" align="center"| '''Product''' || diguanylate cyclase and potential phosphodiesterase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of c-di-GMP |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13420 dgcW] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13420 dgcW] | ||
| Line 66: | Line 66: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** synthesis of c-di-GMP from two molecules of GTP {{PubMed|23893111}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
| Line 76: | Line 77: | ||
* '''Domains:''' | * '''Domains:''' | ||
| + | ** contains a N-terminal PAS domain {{PubMed|23893111}} | ||
** contains a central GGDEF domain and a C-terminal EAL domain {{PubMed|22821967}} | ** contains a central GGDEF domain and a C-terminal EAL domain {{PubMed|22821967}} | ||
** aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity) | ** aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity) | ||
Revision as of 11:09, 10 August 2013
- Description: diguanylate cyclase and potential phosphodiesterase
| Gene name | dgcW |
| Synonyms | ykoW |
| Essential | no |
| Product | diguanylate cyclase and potential phosphodiesterase |
| Function | synthesis of c-di-GMP |
| Gene expression levels in SubtiExpress: dgcW | |
| MW, pI | 84 kDa, 6.823 |
| Gene length, protein length | 2247 bp, 749 aa |
| Immediate neighbours | ykoV, ykoX |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
membrane proteins, metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13420
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-GMP from two molecules of GTP PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34311
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP850 (ykoW::cat), available in Jörg Stülke's lab
- BP140 (ykoW::ermC) available in Fabian Commichau's lab
- BP142 (ykoW-ykoV-ligD::kan) available in Fabian Commichau's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
