IspD

• Description: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, third step in the MEP pathway of isoprenoid biosynthesis
  
  

Gene name	ispD
Synonyms	yacM
Essential	yes PubMed
Product	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Function	MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispD
MW, pI	25 kDa, 5.579
Gene length, protein length	696 bp, 232 aa
Immediate neighbours	yacL, ispF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigB regulon, SigM regulon

The gene

Basic information

• Locus tag: BSU00900

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00900

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)

• Protein family: ispD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU00900

• Structure: 2YC3 (from Arabidopsis thaliana, 38% identity) PubMed

• UniProt: Q06755

• KEGG entry: [3]

• E.C. number: 2.7.7.60

Expression and regulation

• Operon: yacL-ispD-ispF PubMed

• Expression browser: ispD PubMed

• Sigma factor: SigB PubMed, SigM PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410] [WorldCat.org] [DOI] (I e)

Matthias C Witschel, H Wolfgang Höffken, Michael Seet, Liliana Parra, Thomas Mietzner, Frank Thater, Ricarda Niggeweg, Franz Röhl, Boris Illarionov, Felix Rohdich, Johannes Kaiser, Markus Fischer, Adelbert Bacher, François Diederich
Inhibitors of the herbicidal target IspD: allosteric site binding.
Angew Chem Int Ed Engl: 2011, 50(34);7931-5
[PubMed:21766403] [WorldCat.org] [DOI] (I p)

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)