Difference between revisions of "FabI"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11720 fabI]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11720 fabI]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=fabI fabI]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.605   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.605   

Revision as of 10:32, 7 January 2014

  • Description: enoyl-acyl carrier protein reductase

Gene name fabI
Synonyms yjbW
Essential no
Product enoyl-acyl carrier protein reductase
Function fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabI
Metabolic function and regulation of this protein in SubtiPathways:
fabI
MW, pI 27 kDa, 5.605
Gene length, protein length 774 bp, 258 aa
Immediate neighbours thiD, cotO
Sequences Protein DNA DNA_with_flanks
Genetic context
FabI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FabI expression.png




























Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

  • Locus tag: BSU11720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)
  • Protein family: FabI subfamily (according to Swiss-Prot)
  • Paralogous protein(s): FabL, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • inhibited by triclosan PubMed

Database entries

  • Structure: 3OIF (complex with NAD and inhibitor triclosan) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • induced upon fatty acid biosynthesis inhibition PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications