Tgl

• Description: spore associated transglutaminase
  
  

Gene name	tgl
Synonyms	yuxF, yugV
Essential	no
Product	spore associated transglutaminase
Function	introduction of crosslinks in the spore coat protein GerQ
Gene expression levels in SubtiExpress: tgl
MW, pI	28 kDa, 6.687
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	mcpB, yuzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU31270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH₃ (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed

• Protein family: bacillus TGase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P40746

• KEGG entry: [3]

• E.C. number: 2.3.2.13

Expression and regulation

• Operon: tgl PubMed

• Expression browser: tgl PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S122 ( tgl::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage