BslA

• Description: amphiphilic protein, forms water-repellent surface layer of the biofilm, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation
  
  

Gene name	bslA
Synonyms	yuaB, sivB
Essential	no
Product	biofilm surface layer, inhibitor of KinA autophosphorylation
Function	biofilm formation, control of entry into sporulation via the phosphorelay
Gene expression levels in SubtiExpress: bslA
MW, pI	19 kDa, 9.987
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	gbsR, ktrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation, phosphorelay

This gene is a member of the following regulons

AbrB regulon, DegU regulon

The gene

Basic information

• Locus tag: BSU31080

Phenotypes of a mutant

• reduced colony complexity PubMed
• loss of surface repellency of the biofilm PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • inhibits KinA autophophorylation PubMed

• Protein family:

• Paralogous protein(s): SivA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed
  • biofilm matrix in an extracellular polysaccharide-dependent manner PubMed
  • forms a layer on the biofilm surface PubMed

Database entries

• Structure:

• UniProt: P71014

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bslA PubMed

• Expression browser: yuaB PubMed

• Sigma factor:

• Regulation:
  • expression is down-regulated in a rok mutant (indirect effect) PubMed

• Regulatory mechanism:
  • DegU-P: transcription activation PubMed, this might be indirect PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • B. subtilis W939 yuaB::Cm (ATCC6051 based) PubMed
  • B. subtilis NRS2097 yuaB::Cm (NCIB3610 based) PubMed
  • B. subtilis yuaB::Cm (168 based) PubMed

• Expression vector:
  • Physpank-yuaB based on vector pDR111: pDRyuaB2 PubMed

• lacZ fusion:
  • pNW500 PyuaB-lacZ fusion in pDG1663 PubMed

• GFP fusion:
  • PyuaB-gfp fusion in pSG1151 vector: pSGyuaB PubMed

• two-hybrid system:

• Antibody:

References

Original publications

Additional publications: PubMed