Difference between revisions of "YwkE"
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkF]]'', ''[[prfA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywkF]]'', ''[[prfA]]'' | ||
|- | |- | ||
| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37000 | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37000 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37000 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37000 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ywkE_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ywkE_context.gif]] | ||
Revision as of 11:30, 14 May 2013
- Description: similar to N5-glutamine methyltransferase that modifies peptide release factors
| Gene name | ywkE |
| Synonyms | |
| Essential | no |
| Product | N5-glutamine methyltransferase |
| Function | peptide release factor methylation |
| Gene expression levels in SubtiExpress: ywkE | |
| MW, pI | 32 kDa, 4.89 |
| Gene length, protein length | 864 bp, 288 aa |
| Immediate neighbours | ywkF, prfA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, protein modification
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37000
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: hemK family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P45873
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Heidi L Schubert, John D Phillips, Christopher P Hill
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.
Biochemistry: 2003, 42(19);5592-9
[PubMed:12741815]
[WorldCat.org]
[DOI]
(P p)
Valérie Heurgué-Hamard, Stéphanie Champ, Ake Engström, Måns Ehrenberg, Richard H Buckingham
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
EMBO J: 2002, 21(4);769-78
[PubMed:11847124]
[WorldCat.org]
[DOI]
(P p)
Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Simon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada, Hachiro Inokuchi
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
Proc Natl Acad Sci U S A: 2002, 99(3);1473-8
[PubMed:11805295]
[WorldCat.org]
[DOI]
(P p)
