Difference between revisions of "PWH844"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
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| − | vector for | + | pWH844 |
| − | constructed in the | + | |
| + | vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein | ||
| + | |||
| + | the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
| + | |||
| + | constructed in the [[Hillen]] lab | ||
| + | |||
in E. coli: ampicillin resistance | in E. coli: ampicillin resistance | ||
| − | + | ||
| − | + | Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the ''Bacillus subtilis ptsGHI'' operon by glucose is controlled by a novel antiterminator, ''GlcT Mol Microbiol.'' '''25:''' 65-78. [http://www.ncbi.nlm.nih.gov/sites/entrez/11902727 PubMed] | |
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Revision as of 10:12, 15 January 2009
pWH844
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed
