DnaI

• Description: primosome component (helicase loader), part of the replisome
  
  

Gene name	dnaI
Synonyms	ytxA, dnaY
Essential	yes PubMed
Product	primosome component (helicase loader)
Function	DNA replication
Gene expression levels in SubtiExpress: dnaI
Interactions involving this protein in SubtInteract: DnaI
MW, pI	35 kDa, 5.743
Gene length, protein length	933 bp, 311 aa
Immediate neighbours	ytxB, dnaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU28980

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-DnaI PubMed, DnaC-DnaI PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization: cell membrane (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed forms foci, close to oriC PubMed

Database entries

• Structure: 2K7R (N-terminal domain)

• UniProt: P06567

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dnaI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)