DacB

• Description: sporulation-specific penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacB
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU23190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: modifies degree of cross-linking of glycan strands in peptidoglycan

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 3MFD (beta-lactamase superfamily domain)

• UniProt: P35150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacB-spmA-spmB PubMed

• Expression browser: dacB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A744 ( dacB::cat), PubMed, available at BGSC
  • 1A745 ( dacB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: