Difference between revisions of "HolA"
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| − | * '''Description:''' DNA polymerase III, delta subunit, part of the [[replisome]] <br/><br/> | + | * '''Description:''' DNA polymerase III, delta subunit, part of the clamp-loader complex and the [[replisome]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 83: | Line 83: | ||
* '''Domains:''' | * '''Domains:''' | ||
| + | ** domain I: aa 1 - 143, RecA-like fold {{PubMed|23525462}} | ||
| + | ** domain II: aa 144 - 214, four alpha helices {{PubMed|23525462}} | ||
| + | ** domain III: aa 215 - 339, seven alpha helices {{PubMed|23525462}} | ||
* '''Modification:''' | * '''Modification:''' | ||
| Line 98: | Line 101: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3ZH9 3ZH9] {{PubMed|23525462}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54459 P54459] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P54459 P54459] | ||
Revision as of 18:21, 27 March 2013
- Description: DNA polymerase III, delta subunit, part of the clamp-loader complex and the replisome
| Gene name | holA |
| Synonyms | yqeN |
| Essential | yes PubMed |
| Product | DNA polymerase III, delta subunit |
| Function | DNA replication |
| Gene expression levels in SubtiExpress: holA | |
| Interactions involving this protein in SubtInteract: HolA | |
| MW, pI | 40 kDa, 6.231 |
| Gene length, protein length | 1041 bp, 347 aa |
| Immediate neighbours | rpsT, yqzM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25560
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- required for bacteriophage SPP1 replication PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: Nucleoid (Mid-cell) PubMed
Database entries
- UniProt: P54459
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
José P Afonso, Kiran Chintakayala, Chatrudee Suwannachart, Svetlana Sedelnikova, Kevin Giles, John B Hoyes, Panos Soultanas, John B Rafferty, Neil J Oldham
Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase.
Nucleic Acids Res: 2013, 41(9);5115-26
[PubMed:23525462]
[WorldCat.org]
[DOI]
(I p)
Elena M Seco, John C Zinder, Carol M Manhart, Ambra Lo Piano, Charles S McHenry, Silvia Ayora
Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro.
Nucleic Acids Res: 2013, 41(3);1711-21
[PubMed:23268446]
[WorldCat.org]
[DOI]
(I p)
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537]
[WorldCat.org]
[DOI]
(P p)
