SpoIVFB

• Description: intramembrane metalloprotease, processing of pro-sigma-K to active SigK
  
  

Gene name	spoIVFB
Synonyms
Essential	no
Product	intramembrane metalloprotease
Function	processing of pro-sigma-K to active SigK
Gene expression levels in SubtiExpress: spoIVFB
Interactions involving this protein in SubtInteract: SpoIVFB
MW, pI	33 kDa, 8.483
Gene length, protein length	864 bp, 288 aa
Immediate neighbours	rplU, spoIVFA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, proteolysis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU27970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: processing of pro-sigma-K to active SigK PubMed

• Protein family: peptidase M50B family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal cystathionine-beta-synthase (CBS) domain, this domain binds ATP PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity: ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level PubMed

• Interactions:
  • SpoIVFB-proSigK PubMed

• Localization:
  • cell membrane

Database entries

• Structure:

• UniProt: P26937

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIVFA-spoIVFB PubMed

• Expression browser: spoIVFB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Gu Chen, Xu Zhang
New insights into S2P signaling cascades: regulation, variation, and conservation.
Protein Sci: 2010, 19(11);2015-30
[PubMed:20836086] [WorldCat.org] [DOI] (I p)