Difference between revisions of "TenI"
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11660 tenI] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tenA]]'', ''[[thiO]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tenA]]'', ''[[thiO]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11660 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:tenI_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:tenI_context.gif]] | ||
Revision as of 12:33, 13 May 2013
- Description: thiazole tautomerase
| Gene name | tenI |
| Synonyms | |
| Essential | no |
| Product | thiazole tautomerase |
| Function | biosynthesis of thiamine |
| Gene expression levels in SubtiExpress: tenI | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 22 kDa, 8.132 |
| Gene length, protein length | 615 bp, 205 aa |
| Immediate neighbours | tenA, thiO |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11660
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: TMP-PPase family (according to Swiss-Prot)
- Paralogous protein(s): ThiE
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1YAD
- UniProt: P25053
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Tadhg P Begley, Steven E Ealick, Fred W McLafferty
Thiamin biosynthesis: still yielding fascinating biological chemistry.
Biochem Soc Trans: 2012, 40(3);555-60
[PubMed:22616866]
[WorldCat.org]
[DOI]
(I p)
T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260]
[WorldCat.org]
[DOI]
(P p)
Original publications
Amrita B Hazra, Ying Han, Abhishek Chatterjee, Yang Zhang, Rung-Yi Lai, Steven E Ealick, Tadhg P Begley
A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase.
J Am Chem Soc: 2011, 133(24);9311-9
[PubMed:21534620]
[WorldCat.org]
[DOI]
(I p)
Angela V Toms, Amy L Haas, Joo-Heon Park, Tadhg P Begley, Steven E Ealick
Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II.
Biochemistry: 2005, 44(7);2319-29
[PubMed:15709744]
[WorldCat.org]
[DOI]
(P p)
A S Pang, S Nathoo, S L Wong
Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
J Bacteriol: 1991, 173(1);46-54
[PubMed:1898926]
[WorldCat.org]
[DOI]
(P p)
