Difference between revisions of "PpaC"

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|style="background:#ABCDEF;" align="center"|'''Function''' || recovery of phosphate ions from pyrophosphate  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || recovery of phosphate ions from pyrophosphate  
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU40550 ppaC]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU40550 ppaC]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.513   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 4.513   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hypR]]'', ''[[yybP]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hypR]]'', ''[[yybP]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16092&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU40550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU40550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU40550 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ppaC_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ppaC_context.gif]]

Revision as of 14:19, 13 May 2013

  • Description: inorganic pyrophosphatase

Gene name ppaC
Synonyms yybQ
Essential yes PubMed
Product inorganic pyrophosphatase
Function recovery of phosphate ions from pyrophosphate
Gene expression levels in SubtiExpress: ppaC
MW, pI 33 kDa, 4.513
Gene length, protein length 927 bp, 309 aa
Immediate neighbours hypR, yybP
Sequences Protein DNA Advanced_DNA
Genetic context
PpaC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

phosphate metabolism, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40550

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Diphosphate + H2O = 2 phosphate (according to Swiss-Prot)
  • Protein family: PPase class C family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • S-cysteinylation after diamide stress (C158) PubMed
    • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: ppaC (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

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