Difference between revisions of "AnsR"
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|style="background:#ABCDEF;" align="center"|'''Function''' || negative regulation of the ''[[ansA]]-[[ansB]]'' operon | |style="background:#ABCDEF;" align="center"|'''Function''' || negative regulation of the ''[[ansA]]-[[ansB]]'' operon | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23590 ansR] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansA]]'', ''[[yqxK]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansA]]'', ''[[yqxK]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23590 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23590 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23590 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:ansR_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ansR_context.gif]] | ||
Revision as of 13:13, 13 May 2013
| Gene name | ansR |
| Synonyms | |
| Essential | no |
| Product | transcriptional regulator (Xre family) |
| Function | negative regulation of the ansA-ansB operon |
| Gene expression levels in SubtiExpress: ansR | |
| Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn | |
| MW, pI | 13 kDa, 4.807 |
| Gene length, protein length | 348 bp, 116 aa |
| Immediate neighbours | ansA, yqxK |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of amino acids, transcription factors and their control
This gene is a member of the following regulons
The AnsR regulon:
The gene
Basic information
- Locus tag: BSU23590
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: Q07683
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ansR PubMed
- Sigma factor:
- Additional information:
Biological materials
- Mutant: GP1152 (del tet) available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666]
[WorldCat.org]
[DOI]
(I e)
Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346]
[WorldCat.org]
[DOI]
(P p)
D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318]
[WorldCat.org]
[DOI]
(P p)
