TatCD

• Description: component of the TatAdCd twin-arginine translocase
  
  

Gene name	tatCD
Synonyms	ycbT
Essential	no
Product	component of the twin-arginine translocation pathway
Function	TAT protein secretion
Gene expression levels in SubtiExpress: tatCD
Interactions involving this protein in SubtInteract: TatCD
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	27 kDa, 9.342
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	tatAD, pcp
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, protein secretion, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU02640

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: translocation of PhoD PubMed

• Protein family: tatC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TatAD-TatCD PubMed
  • TatAC-TatCD PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P42252

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: tatCD PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

References

Reviews

Tracy Palmer, Ben C Berks
The twin-arginine translocation (Tat) protein export pathway.
Nat Rev Microbiol: 2012, 10(7);483-96
[PubMed:22683878] [WorldCat.org] [DOI] (I e)

Original publications

Carmine G Monteferrante, Jacopo Baglieri, Colin Robinson, Jan Maarten van Dijl
TatAc, the third TatA subunit of Bacillus subtilis, can form active twin-arginine translocases with the TatCd and TatCy subunits.
Appl Environ Microbiol: 2012, 78(14);4999-5001
[PubMed:22544248] [WorldCat.org] [DOI] (I p)

Olga V Nolandt, Torsten H Walther, Siegmar Roth, Jochen Bürck, Anne S Ulrich
Structure analysis of the membrane protein TatC(d) from the Tat system of B. subtilis by circular dichroism.
Biochim Biophys Acta: 2009, 1788(10);2238-44
[PubMed:19616508] [WorldCat.org] [DOI] (P p)

Anja N J A Ridder, Esther J de Jong, Jan D H Jongbloed, Oscar P Kuipers
Subcellular localization of TatAd of Bacillus subtilis depends on the presence of TatCd or TatCy.
J Bacteriol: 2009, 191(13);4410-8
[PubMed:19395490] [WorldCat.org] [DOI] (I p)

Robyn T Eijlander, Magdalena A Kolbusz, Erwin M Berendsen, Oscar P Kuipers
Effects of altered TatC proteins on protein secretion efficiency via the twin-arginine translocation pathway of Bacillus subtilis.
Microbiology (Reading): 2009, 155(Pt 6);1776-1785
[PubMed:19383693] [WorldCat.org] [DOI] (P p)

Jan D H Jongbloed, Ulrike Grieger, Haike Antelmann, Michael Hecker, Reindert Nijland, Sierd Bron, Jan Maarten van Dijl
Two minimal Tat translocases in Bacillus.
Mol Microbiol: 2004, 54(5);1319-25
[PubMed:15554971] [WorldCat.org] [DOI] (P p)

J D Jongbloed, U Martin, H Antelmann, M Hecker, H Tjalsma, G Venema, S Bron, J M van Dijl, J Müller
TatC is a specificity determinant for protein secretion via the twin-arginine translocation pathway.
J Biol Chem: 2000, 275(52);41350-7
[PubMed:11007775] [WorldCat.org] [DOI] (P p)