PpaC

• Description: inorganic pyrophosphatase
  
  

Gene name	ppaC
Synonyms	yybQ
Essential	yes PubMed
Product	inorganic pyrophosphatase
Function	recovery of phosphate ions from pyrophosphate
Gene expression levels in SubtiExpress: ppaC
MW, pI	33 kDa, 4.513
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	hypR, yybP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

phosphate metabolism, essential genes

The gene

Basic information

• Locus tag: BSU40550

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Diphosphate + H₂O = 2 phosphate (according to Swiss-Prot)

• Protein family: PPase class C family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • S-cysteinylation after diamide stress (C158) PubMed
  • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM

• UniProt: P37487

• KEGG entry: [3]

• E.C. number: 3.6.1.1

Expression and regulation

• Operon: ppaC (according to DBTBS)

• Expression browser: ppaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: