Difference between revisions of "FolD"
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|style="background:#ABCDEF;" align="center"|'''Function''' || formylation of Met-tRNA(fMet) | |style="background:#ABCDEF;" align="center"|'''Function''' || formylation of Met-tRNA(fMet) | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24310 folD] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.6 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30 kDa, 5.6 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiB]]'', ''[[nusB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqiB]]'', ''[[nusB]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24310 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24310 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24310 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:folD_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:folD_context.gif]] | ||
Revision as of 13:15, 13 May 2013
- Description: methylenetetrahydrofolate dehydrogenase (NADP)
| Gene name | folD |
| Synonyms | yqiA |
| Essential | yes PubMed |
| Product | methylenetetrahydrofolate dehydrogenase (NADP) |
| Function | formylation of Met-tRNA(fMet) |
| Gene expression levels in SubtiExpress: folD | |
| MW, pI | 30 kDa, 5.6 |
| Gene length, protein length | 849 bp, 283 aa |
| Immediate neighbours | yqiB, nusB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24310
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH (according to Swiss-Prot)
- Protein family: tetrahydrofolate dehydrogenase/cyclohydrolase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54382
- KEGG entry: [3]
- E.C. number: 1.5.1.5
Additional information
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yann Duroc, Carmela Giglione, Thierry Meinnel
Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.
Antimicrob Agents Chemother: 2009, 53(4);1673-8
[PubMed:19171795]
[WorldCat.org]
[DOI]
(I p)
H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660]
[WorldCat.org]
[DOI]
(P p)
