Difference between revisions of "Nth"
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
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| + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nth_2344755_2345414_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:nth_expression.png|500px]] | ||
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Revision as of 11:56, 19 April 2012
- Description: endonuclease III, removes abasic sites during base excision repair
| Gene name | nth |
| Synonyms | jooB, ypoB |
| Essential | no |
| Product | endonuclease III |
| Function | DNA repair |
| MW, pI | 24 kDa, 9.277 |
| Gene length, protein length | 657 bp, 219 aa |
| Immediate neighbours | ypoC, dnaD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22340
Phenotypes of a mutant
- the mutant is sensititive to exposure to H2O2 PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1P59 (complex with DNA, Geobacillus stearothermophilus)
- UniProt: P39788
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Christopher Collier, Cristina Machón, Geoff S Briggs, Wiep Klaas Smits, Panos Soultanas
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Nucleic Acids Res: 2012, 40(2);739-50
[PubMed:21954439]
[WorldCat.org]
[DOI]
(I p)
J Christopher Fromme, Gregory L Verdine
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J: 2003, 22(13);3461-71
[PubMed:12840008]
[WorldCat.org]
[DOI]
(P p)
