Difference between revisions of "SsbB"

From SubtiWiki
Jump to: navigation, search
Line 82: Line 82:
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** forms tetramers {{PubMed|22373918}}
 
** [[SsbB]]-[[ComFA]] {{PubMed|17630974}}
 
** [[SsbB]]-[[ComFA]] {{PubMed|17630974}}
 
** [[SsbB]]-[[CoiA]] {{PubMed|17630974}}
 
** [[SsbB]]-[[CoiA]] {{PubMed|17630974}}
Line 87: Line 88:
 
** [[SsbB]]-[[RecA]] {{PubMed|17630974}}
 
** [[SsbB]]-[[RecA]] {{PubMed|17630974}}
  
* '''[[Localization]]:''' localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
+
* '''[[Localization]]:'''  
 +
** localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId= 3VDY 3VDY] (in complex with ssDNA) {{PubMed|22373918}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SPB6 C0SPB6]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/C0SPB6 C0SPB6]

Revision as of 13:42, 6 March 2012

  • Description: single-strand DNA-binding protein

Gene name ssbB
Synonyms ywpH
Essential no
Product single-strand DNA-binding protein
Function required for efficient genetic transformation
Interactions involving this protein in SubtInteract: SsbB
MW, pI 12 kDa, 6.876
Gene length, protein length 339 bp, 113 aa
Immediate neighbours glcR, ywpG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwpH context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA replication, DNA repair/ recombination

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU36310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): SsbA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at the onset of stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References