Difference between revisions of "Methods"
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* ''in vivo'' detection of [[protein-protein interactions]] using [[DivIVA]] and GFP {{PubMed|19698693}} | * ''in vivo'' detection of [[protein-protein interactions]] using [[DivIVA]] and GFP {{PubMed|19698693}} | ||
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| + | ==Analysis of membrane proteins== | ||
| + | * application of a split green fluorescent protein reporter {{PubMed|22285978}} | ||
==Strain construction== | ==Strain construction== | ||
Revision as of 18:22, 9 February 2012
Here you can find a list of methods for working with Bacillus subtilis:
Contents
Analysis of protein-protein interactions
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- in vivo detection of protein-protein interactions using DivIVA and GFP PubMed
Analysis of membrane proteins
- application of a split green fluorescent protein reporter PubMed
Strain construction
- introduction of markerfree deletions: PubMed
RNA analysis
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
Work with non-domesticated strains
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
General procedures
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
Live cell imaging
- Live cell imaging of B. subtilis cells using automated time-lapse microscopy PubMed
- Assay of gene expression dynamics using live cell imaging PubMed
Transposon mutagenesis
- mariner transposon mutagenesis for random inducible-expression insertions and transcriptional reporter fusion insertions PubMed
Protein-DNA interactions
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
Key references
Additional publications: PubMed
