AsnO

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnO
Synonyms	yisO, yucB
Essential	no
Product	asparagine synthase (glutamine-hydrolysing)
Function	biosynthesis of asparagine
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	70 kDa, 5.696
Gene length, protein length	1842 bp, 614 aa
Immediate neighbours	yisN, yizA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU10790

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O05272

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: asnO PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation (SigE) PubMed
  • subject to positive regulation by SpoIIID PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)