YwjH

• Description: transaldolase
  
  

Gene name	ywjH
Synonyms
Essential	no
Product	transaldolase
Function	pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	22 kDa, 5.876
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	murAB, fbaA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

The gene

Basic information

• Locus tag: BSU37110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: Type 3B subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms decamers (dimer of pentamers) PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1WX0 (from Thermus thermophilus hb8, 50% identity, 65% similarity)

• UniProt: P19669

• KEGG entry: [3]

• E.C. number: 2.2.1.2

Expression and regulation

• Operon: fbaA-ywjH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Stülke lab
  • pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Stülke lab
  • GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1407 (spc, based on pGP1331), available in the Stülke lab