YmdB

• Description: putative phosphatase/ phosphodiesterase, controls bistable gene expression
  
  

Gene name	ymdB
Synonyms
Essential	no
Product	putative phosphatase/ phosphodiesterase
Function	control of bistable gene expression
MW, pI	29,1 kDa, 6.50
Gene length, protein length	792 bp, 264 amino acids
Immediate neighbours	rny, spoVS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biofilm formation

The gene

Basic information

• Locus tag: BSU16970

Phenotypes of a mutant

• strong overexpression of hag PubMed
• defective in biofilm formation PubMed
• the phenotypes of the ymdB mutant can be suppressed by overexpression of slrR PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: phosphatase activity toward phosphoenolpyruvate and phosphodiesterase activity toward 2',3'-cAMP (the Deinococcus ortholog) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1T70 (the protein of Deinococcus radiodurans, 44% identity) PubMed

• UniProt: O31775

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rny-ymdB (weak readthrough transcription) PubMed

• Sigma factor:

• Regulation: constitutive

• Regulatory mechanism:

• Additional information: there is a terminator between rny and ymdB, most transcripts terminate there PubMed

Biological materials

• Mutant:
  • GP583 (spc), available in Stülke lab
  • GP922 (cat), available in Stülke lab
  • GP921 (spc) NCIB3610 derivate, available in Stülke lab
  • GP969 (ymdB(E39Q)-cat) inactive enzyme, available in Stülke lab

• Expression vector:
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP1041, available in Stülke lab
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1919, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1040, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal Strep-tag, in pGP172: pGP1917, available in Stülke lab
  • GP970 (ymdB-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1018 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

References

Eric R Pozsgai, Kris M Blair, Daniel B Kearns
Modified mariner transposons for random inducible-expression insertions and transcriptional reporter fusion insertions in Bacillus subtilis.
Appl Environ Microbiol: 2012, 78(3);778-85
[PubMed:22113911] [WorldCat.org] [DOI] (I p)

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J    
A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects
Flagellin Expression and Biofilm Formation. 
J Bacteriol.: 2011, 193(21):5997-6007. 
PubMed:21856853