Nfo

• Description: type IV apurinic/apyrimidinic endonuclease
  
  

Gene name	nfo
Synonyms	yqfS
Essential	no
Product	type IV apurinic/apyrimidinic endonuclease
Function	repair of oxidative DNA damage in spores
MW, pI	32 kDa, 5.371
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	yqfT, cshB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU25130

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• Nfo is functionally redundant with ExoA

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products (according to Swiss-Prot)

• Protein family: AP endonuclease 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54476

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfo-yqfU PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

The gene yqfT is located between nfo and yqfU, but is transcribed in the opposite direction.

Biological materials

• Mutant:
  • available in Mario Pedraza-Reyes' lab
  • GP899 (nfo::kan) and GP1502 (nfo::cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed