Difference between revisions of "FbaA"
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=== Basic information === | === Basic information === | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
Revision as of 11:54, 13 January 2009
- Description: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis
| Gene name | fbaA |
| Synonyms | fba, fba1, tsr |
| Essential | yes |
| Product | fructose-1,6-bisphosphate aldolase |
| Function | aldol condensation & reverse reaction |
| MW, pI | 30,2 kDa, 5.03 |
| Gene length, protein length | 855 bp, 285 amino acids |
| Immediate neighbours | spo0F, ywjH |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates: 3807538 - 3808392
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
- Protein family: class II fructose-bisphosphate aldolase family.
- Paralogous protein(s): FbaB
Extended information on the protein
- Kinetic information:
- Domains:
- 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
- Modification: Phosphorylated during sporulation
- Cofactor(s): 2 x zinc ion
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: [3]
- KEGG entry: [4]
- E.C. number: [5]
Additional information
Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
Expression and regulation
- Operon:
- fbaA
- fbaA-ywjH
- Sigma factor:
- Regulation: constitutively expressed
- Regulatory mechanism:
- Additional information:
Biological materials
Labs working on this gene/protein
Your additional remarks
References
- Trach K, Chapman JW & Piggot P (1988) Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome J Bacteriol. 170: 4194-4208. PubMed
- Ludwig H, Homuth G & Schmalisch M (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon Mol Microbiol. 41: 409-22. PubMed
