SlrR

• Description: transcriptional activator of competence development and sporulation genes, represses SigD-dependent flagellar genes, antagonist of SlrA and SinR, has LexA-like autocleavage activity
  
  

Gene name	slrR
Synonyms	yveJ, slr
Essential	no
Product	transcription regulator, SlrA antagonist
Function	regulation of initiation of biofilm formation and of autolysis
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	17 kDa, 9.63
Gene length, protein length	456 bp, 152 aa
Immediate neighbours	epsA, pnbA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

Abh regulon, SinR regulon

The SlrR regulon:

• lytA-lytB-lytC, lytF

The gene

Basic information

• Locus tag: BSU34380

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • SlrR binds to and inhibits the activity of SlrA, SlrA indirectly stimulates the synthesis of SlrR by interacting with SinR. SlrR can bind to SinR and SinR directly represses the transcription of SlrR. SlrR indirectly derepresses its own gene. The heterocomplex of SlrR-SinR is a repressor of autolysin and motility genes and inhibits the repressor function of SinR. PubMed
  • repression of transcription of lytA-lytB-lytC and lytF PubMed
  • autocleavage PubMed

• Protein family:

• Paralogous protein(s): SinR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • subject to self-cleavage via a LexA-like autopeptidase, this involves ClpC PubMed

• Cofactor(s):

• Effectors of protein activity: interaction with SinR triggers binding of SlrR to the promoters of lytA-lytB-lytC and lytF, resulting in their repression PubMed

• Interactions:
  • SlrA-SlrR PubMed
  • SinR-SlrR PubMed

• Localization:

Database entries

• Structure:

• UniProt: P71049

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • Abh: transcription activation PubMed
  • SinR: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI, SlrA PubMed or by SlrR itself PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Patrick Piggot
Epigenetic switching: bacteria hedge bets about staying or moving.
Curr Biol: 2010, 20(11);R480-2
[PubMed:20541494] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed