Difference between revisions of "SubtInteract"
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==Methods to detect protein-protein interactions== | ==Methods to detect protein-protein interactions== | ||
| − | * Bacterial Two Hybrid System: [[BACTH]] | + | * Bacterial Two Hybrid System: [[BACTH]] {{PubMed|9576956}} |
* Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}} | * Yeast Two Hybrid System {{PubMed|18218650,16806892,16262681,15064463,11769011,10967325,8816737,7894083}} | ||
| − | * [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking | + | * [[SPINE]]: A method to detect ''in vivo'' protein-protein interactions after cross-linking {{PubMed|17994626}} |
* TAP-Tag purification {{PubMed|10504710,11403571}} | * TAP-Tag purification {{PubMed|10504710,11403571}} | ||
* ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}} | * ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}} | ||
| + | |||
| + | '''Attention:''' Each technique detects only about 33% of all interactions {{PubMed|10504710,11403571}} | ||
==Important publications== | ==Important publications== | ||
<pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 </pubmed> | <pubmed>20658969 18228443 18219467 17953394 15264234 14706816 12127457 11827824 11377797 11306254 10851163 10842303 </pubmed> | ||
Revision as of 21:15, 3 January 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Finally, many interactions may be of a transient nature.
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Important publications
Mike P Williamson, Michael J Sutcliffe
Protein-protein interactions.
Biochem Soc Trans: 2010, 38(4);875-8
[PubMed:20658969]
[WorldCat.org]
[DOI]
(I p)
Adam Brymora, Valentina A Valova, Phillip J Robinson
Protein-protein interactions identified by pull-down experiments and mass spectrometry.
Curr Protoc Cell Biol: 2004, Chapter 17;Unit 17.5
[PubMed:18228443]
[WorldCat.org]
[DOI]
(I p)
Hongtao Guan, Endre Kiss-Toth
Advanced technologies for studies on protein interactomes.
Adv Biochem Eng Biotechnol: 2008, 110;1-24
[PubMed:18219467]
[WorldCat.org]
[DOI]
(I p)
Vincent Collura, Guillaume Boissy
From protein-protein complexes to interactomics.
Subcell Biochem: 2007, 43;135-83
[PubMed:17953394]
[WorldCat.org]
[DOI]
(P p)
Barry Causier
Studying the interactome with the yeast two-hybrid system and mass spectrometry.
Mass Spectrom Rev: 2004, 23(5);350-67
[PubMed:15264234]
[WorldCat.org]
[DOI]
(P p)
Andrzej Dziembowski, Bertrand Séraphin
Recent developments in the analysis of protein complexes.
FEBS Lett: 2004, 556(1-3);1-6
[PubMed:14706816]
[WorldCat.org]
[DOI]
(P p)
Alfonso Valencia, Florencio Pazos
Computational methods for the prediction of protein interactions.
Curr Opin Struct Biol: 2002, 12(3);368-73
[PubMed:12127457]
[WorldCat.org]
[DOI]
(P p)
Peter Uetz
Two-hybrid arrays.
Curr Opin Chem Biol: 2002, 6(1);57-62
[PubMed:11827824]
[WorldCat.org]
[DOI]
(P p)
P Legrain, J Wojcik, J M Gauthier
Protein--protein interaction maps: a lead towards cellular functions.
Trends Genet: 2001, 17(6);346-52
[PubMed:11377797]
[WorldCat.org]
[DOI]
(P p)
C L Tucker, J F Gera, P Uetz
Towards an understanding of complex protein networks.
Trends Cell Biol: 2001, 11(3);102-6
[PubMed:11306254]
[WorldCat.org]
[DOI]
(P p)
P Uetz, R E Hughes
Systematic and large-scale two-hybrid screens.
Curr Opin Microbiol: 2000, 3(3);303-8
[PubMed:10851163]
[WorldCat.org]
[DOI]
(P p)
Z Chen, M Han
Building a protein interaction map: research in the post-genome era.
Bioessays: 2000, 22(6);503-6
[PubMed:10842303]
[WorldCat.org]
[DOI]
(P p)
