MalR

• Description: two-component response regulator, regulation of malate uptake
  
  

Gene name	malR
Synonyms	yufM
Essential	no
Product	two-component response regulator
Function	regulation of malate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	26 kDa, 5.976
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	malK, yufN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

The MalR regulon:

• maeA-ywkB, maeN, yflS

The gene

Basic information

• Locus tag: BSU31530

Phenotypes of a mutant

unable to use malate as the single carbon source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by MalK on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions: MalK-MalR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05251

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: malR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1301 (cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France