Difference between revisions of "YwkE"
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| − | * '''Description:''' similar to | + | * '''Description:''' similar to N5-glutamine methyltransferase that modifies peptide release factors <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || N5-glutamine methyltransferase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || peptide release factor methylation |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 4.89 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 4.89 | ||
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[translation]]}}, |
| + | {{SubtiWiki category|[[protein modification]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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=References= | =References= | ||
| − | + | <pubmed> 11805295 11847124 </pubmed> | |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:44, 1 August 2011
- Description: similar to N5-glutamine methyltransferase that modifies peptide release factors
| Gene name | ywkE |
| Synonyms | |
| Essential | no |
| Product | N5-glutamine methyltransferase |
| Function | peptide release factor methylation |
| MW, pI | 32 kDa, 4.89 |
| Gene length, protein length | 864 bp, 288 aa |
| Immediate neighbours | ywkF, prfA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
translation, protein modification
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37000
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: hemK family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P45873
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Valérie Heurgué-Hamard, Stéphanie Champ, Ake Engström, Måns Ehrenberg, Richard H Buckingham
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.
EMBO J: 2002, 21(4);769-78
[PubMed:11847124]
[WorldCat.org]
[DOI]
(P p)
Kenji Nakahigashi, Naoko Kubo, Shin-ichiro Narita, Takeshi Shimaoka, Simon Goto, Taku Oshima, Hirotada Mori, Maki Maeda, Chieko Wada, Hachiro Inokuchi
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.
Proc Natl Acad Sci U S A: 2002, 99(3);1473-8
[PubMed:11805295]
[WorldCat.org]
[DOI]
(P p)
