Difference between revisions of "GanA"
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| − | * '''Mutant:''' | + | * '''Mutant:'''GP1181 (aphA3) available in the [[Stülke]] lab |
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* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 16:13, 7 February 2011
- Description: beta-galactosidase
| Gene name | lacA |
| Synonyms | yvfN |
| Essential | no |
| Product | beta-galactosidase |
| Function | galactan utilization |
| MW, pI | 79 kDa, 5.704 |
| Gene length, protein length | 2061 bp, 687 aa |
| Immediate neighbours | yvfO, yvfM |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
utilization of specific carbon sources
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34130
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)
- Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O07012
- KEGG entry: [3]
- E.C. number: 3.2.1.23 3
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:GP1181 (aphA3) available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Stephanie Shipkowski, Jean E Brenchley
Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases.
Appl Environ Microbiol: 2006, 72(12);7730-8
[PubMed:17056685]
[WorldCat.org]
[DOI]
(P p)
R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030]
[WorldCat.org]
[DOI]
(P p)
J Errington, C H Vogt
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol: 1990, 172(1);488-90
[PubMed:2104611]
[WorldCat.org]
[DOI]
(P p)
