Difference between revisions of "RnjB"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[RnjA]]-[[RnjB]] {{PubMed|19193632,20025672}} | + | * '''[[Interactions]]:''' part of the [[RNA degradosome]] |
| + | ** [[RnjA]]-[[RnjB]] {{PubMed|19193632,20025672}} | ||
* '''Localization:''' cytoplasm (according to Swiss-Prot) | * '''Localization:''' cytoplasm (according to Swiss-Prot) | ||
Revision as of 08:05, 6 March 2011
- Description: RNase J2
| Gene name | rnjB |
| Synonyms | ymfA |
| Essential | no |
| Product | RNase J2 |
| Function | RNA processing and degradation |
| MW, pI | 56 kDa, 9.18 |
| Gene length, protein length | 1545 bp, 515 aa |
| Immediate neighbours | dapA, tepA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16780
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: endoribonuclease, involved in processing of thrS mRNA
- Protein family: RNase J subfamily (according to Swiss-Prot)
- Paralogous protein(s): RnjA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: part of the RNA degradosome
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 3BK1 (RNase J from Thermus thermophilus) 3BK2 (RNase J from Thermus thermophilus, complex with UMP)
- UniProt: O31760
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP45 (spc), GP1113 (miniTn10 spc), both available in Stülke lab
- Expression vector:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
Your additional remarks
References
Reviews
Original publications
