Difference between revisions of "Mfd"
| Line 57: | Line 57: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''Interactions:''' |
| + | ** [[Mfd]]-[[UvrA]] {{PubMed|16464004}} | ||
| + | ** [[Mfd]]-[[RpoB]] {{PubMed|20702425}} | ||
* '''Localization:''' | * '''Localization:''' | ||
| Line 89: | Line 88: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.proteopedia.org/wiki/index.php/2eyq 2EYQ] (Mfd from ''E. coli'') {{PubMed|16469698}} | + | * '''Structure:''' |
| + | ** [http://www.proteopedia.org/wiki/index.php/2eyq 2EYQ] (Mfd from ''E. coli'') {{PubMed|16469698}} | ||
| + | ** [http://www.pdb.org/pdb/explore/explore.do?structureId=3MLQ 3MLQ] (RNA polymerase interacting domain | ||
| + | of ''Thermus thermophilus'' [[Mfd]] with the ''Thermus aquaticus'' [[RpoB]] beta1 domain) {{PubMed|20702425}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37474 P37474] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P37474 P37474] | ||
| Line 134: | Line 136: | ||
==Original Articles== | ==Original Articles== | ||
| − | <pubmed>8594198,20435731 ,104170,16950921,9535092, 16469698 </pubmed> | + | <pubmed>8594198,20435731 ,104170,16950921,9535092, 16469698 20702425 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:17, 16 December 2010
- Description: transcription-repair coupling factor
| Gene name | mfd |
| Synonyms | |
| Essential | no |
| Product | transcription-repair coupling factor |
| Function | promotes strand-specific DNA repair by displacing
RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site |
| MW, pI | 133 kDa, 5.367 |
| Gene length, protein length | 3531 bp, 1177 aa |
| Immediate neighbours | fin, spoVT |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, transcription
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00550
Phenotypes of a mutant
In an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): RecG
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
- UniProt: P37474
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1167 (ermC), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Rodrigo S Galhardo, P J Hastings, Susan M Rosenberg
Mutation as a stress response and the regulation of evolvability.
Crit Rev Biochem Mol Biol: 2007, 42(5);399-435
[PubMed:17917874]
[WorldCat.org]
[DOI]
(P p)
Alexandra M Deaconescu, Nigel Savery, Seth A Darst
The bacterial transcription repair coupling factor.
Curr Opin Struct Biol: 2007, 17(1);96-102
[PubMed:17239578]
[WorldCat.org]
[DOI]
(P p)
James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004]
[WorldCat.org]
[DOI]
(P p)
Sergei Borukhov, Jookyung Lee, Oleg Laptenko
Bacterial transcription elongation factors: new insights into molecular mechanism of action.
Mol Microbiol: 2005, 55(5);1315-24
[PubMed:15720542]
[WorldCat.org]
[DOI]
(P p)
Jeffrey Roberts, Joo-Seop Park
Mfd, the bacterial transcription repair coupling factor: translocation, repair and termination.
Curr Opin Microbiol: 2004, 7(2);120-5
[PubMed:15063847]
[WorldCat.org]
[DOI]
(P p)
E C Friedberg
Relationships between DNA repair and transcription.
Annu Rev Biochem: 1996, 65;15-42
[PubMed:8811173]
[WorldCat.org]
[DOI]
(P p)
C P Selby, A Sancar
Mechanisms of transcription-repair coupling and mutation frequency decline.
Microbiol Rev: 1994, 58(3);317-29
[PubMed:7968917]
[WorldCat.org]
[DOI]
(P p)
Original Articles
