KinD

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, checkpoint protein that links sporulation initiation to biofilm formation
  
  

Gene name	kinD
Synonyms	ykvD
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	56 kDa, 6.745
Gene length, protein length	1518 bp, 506 aa
Immediate neighbours	eag, mhqR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13660

Phenotypes of a mutant

deletion of kinD suppresses the sporulation defect of matrix mutants, while its overproduction delays sporulation PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F, regulates the onset of sporulation by inhibiting the activity of Spo0A until matrix, or a component therein, is sensed PubMed
  • dual role as a phosphatase or a kinase, activity is linked to the presence of extracellular matrix in the biofilms PubMed
  • mainly active in the younger, outer regions of a colony (with KinC) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions: KinD-Spo0F

• Localization:

Database entries

• Structure: 3FOS

• UniProt: O31671

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: