RocA

• Description: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
  
  

Gene name	rocA
Synonyms	ipa-76d
Essential	no
Product	3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
Function	arginine, ornithine and citrulline utilization
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	56 kDa, 5.58
Gene length, protein length	1545 bp, 515 aa
Immediate neighbours	rocB, rocG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H (according to Swiss-Prot)

• Protein family: RocA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BJA (with bound NADH from Thermus thermophilus, 43% identity, 59% similarity) PubMed

• UniProt: P39634

• KEGG entry: [3]

• E.C. number: 1.5.1.12

Expression and regulation

• Operon: rocA-rocB-rocC PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • induction by arginine (RocR, AhrC) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • RocR: transcription activation PubMed
  • AhrC: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: