LysC

• Description: aspartokinase II (alpha and beta subunits)
  
  

Gene name	lysC
Synonyms	ask, aecA
Essential	no
Product	aspartokinase II (alpha and beta subunits)
Function	biosynthesis of lysine
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr
MW, pI	43 kDa, 4.643
Gene length, protein length	1224 bp, 408 aa
Immediate neighbours	yslB, ask
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate = ADP + 4-phospho-L-aspartate (according to Swiss-Prot)

• Protein family: aspartokinase family (according to Swiss-Prot)

• Paralogous protein(s): DapG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2RE1 (from Neisseria meningitidis mc58, 40% identity, 58% similarity)

• UniProt: P08495

• KEGG entry: [3]

• E.C. number: 2.7.2.4

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed

Expression and regulation

• Operon: lysC PubMed

• Sigma factor:

• Regulation:
  • repressed in the presence of lysine (L-box) PubMed
  • expression activated by glucose (5.4 fold) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • L-box: a riboswich that mediates transcription terimnation antitermination control PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Chien-Chi Lo, Carol A Bonner, Gary Xie, Mark D'Souza, Roy A Jensen
Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.
Microbiol Mol Biol Rev: 2009, 73(4);594-651
[PubMed:19946135] [WorldCat.org] [DOI] (I p)