Difference between revisions of "List of plasmids"

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Here you can find a list of ''Bacillus subtilis'' plasmids:
 
Here you can find a list of ''Bacillus subtilis'' plasmids:
  
*[[pAC5]]
+
*[[pAC5]]: translational ''lacZ''-fusions (chloramphenicol resistence)
*[[pAC6]]
+
*[[pAC6]]: transcriptional ''lacZ''-fusions (cat resistence)
*[[pAC7]]
+
*[[pAC7]]: translational ''lacZ''-fusions (kanamycin resistence)
 +
* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
  
* pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
+
*[[pBQ200]]: overexpression of proteins in ''B. subtilis'' under control of a strong ''degQ36'' promoter
*[[pBQ200]]
+
*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein
 +
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
  
*[[pGP172]]
+
*[[pWH844]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag
 +
*[[pGP570]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag that can be cleaved off
  
*[[pGP380]]
+
*[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein
*[[pGP382]]
+
*[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein
 
 
*[[pGP570]]
 
*[[pGP574]]
 
 
 
*[[pWH844]]
 

Revision as of 11:44, 11 March 2010

Here you can find a list of Bacillus subtilis plasmids:

  • pAC5: translational lacZ-fusions (chloramphenicol resistence)
  • pAC6: transcriptional lacZ-fusions (cat resistence)
  • pAC7: translational lacZ-fusions (kanamycin resistence)
  • pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed
  • pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
  • pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
  • pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
  • pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
  • pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein