Difference between revisions of "PAC6"
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in ''E. coli'': ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
| − | in ''B. subtilis'': | + | in ''B. subtilis'': chloramphenicol resistance |
similar vectors: [[pAC5]], [[pAC7]] | similar vectors: [[pAC5]], [[pAC7]] | ||
Revision as of 14:40, 22 September 2011
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenicol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
