Difference between revisions of "HprK"
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* '''Mutant:''' GP202 (spc), GP858 (aphA3), both available in [[Stülke]] lab | * '''Mutant:''' GP202 (spc), GP858 (aphA3), both available in [[Stülke]] lab | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
Revision as of 17:25, 2 September 2009
- Description: HPr kinase/ phosphorylase
| Gene name | hprK |
| Synonyms | ptsK, yvoB |
| Essential | no |
| Product | HPr kinase/ phosphorylase |
| Function | carbon catabolite repression, phosphorylation of HPr and Crh proteins at Ser46 |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 34 kDa, 4.906 |
| Gene length, protein length | 930 bp, 310 aa |
| Immediate neighbours | lgt, nagA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU35000
Phenotypes of a mutant
no carbon catabolite repression
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + HPr = ADP + P-Ser-HPr (according to Swiss-Prot)
- Protein family: HPrK/P family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 1KKM (complex of Lactobacillus casei HprK with B. subtilis HPr-Ser-P), 1KKL (complex of Lactobacillus casei HprK with B. subtilis HPr)
- UniProt: O34483
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP202 (spc), GP858 (aphA3), both available in Stülke lab
- Expression vector:
- for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP642, available in Stülke lab
- for expression/ purification of mutant HprK-G158A from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP650, available in Stülke lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP205, available in Stülke lab
- for expression, purification of the N-terminal in E. coli with N-terminal His-tag, in pWH844: pGP218, available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody: available in Stülke lab
Labs working on this gene/protein
Josef Deutscher, Paris-Grignon, France
Jörg Stülke, University of Göttingen, Germany Homepage
Wolfgang Hillen, Erlangen University, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
review
general/ physiology
enzymatic properties, mutation analysis
structure analysis
HprK as target for antimicrobial compounds
