Difference between revisions of "AcoA"
| Line 74: | Line 74: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' cytoplasm (homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] |
=== Database entries === | === Database entries === | ||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | |||
* '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | * '''Operon:''' ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | ||
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | ||
| − | * '''Regulation:''' repressed by glucose ([[CcpA]]) | + | * '''Regulation:''' |
| + | ** repressed by glucose ([[CcpA]]) {{PubMed|10666464}} | ||
| + | ** induced by acetoin ([[AcoR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | ||
| + | ** induced under anaerobic conditions ([[Fnr]]) {{PubMed|16428414}} | ||
| − | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression | + | * '''Regulatory mechanism:''' |
| + | ** [[CcpA]]: transcription repression {{PubMed|10666464}} | ||
| + | ** [[AcoR]]: transcription activation (interaction with [[SigL]]-containing RNA polymerase) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed] | ||
| + | ** [[Fnr]]: indirect positive regulation via [[NarG]]-[[NarH]]-[[NarJ]]-[[NarI]] {{PubMed|16428414}} | ||
* '''Additional information:'''the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed] | * '''Additional information:'''the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed] | ||
| Line 122: | Line 127: | ||
=References= | =References= | ||
| − | <pubmed>11274109,10368162,,12884008,16479537,11274109 </pubmed> | + | <pubmed>11274109,10368162,10666464,12884008,16479537,11274109 16428414 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 17:44, 14 May 2010
- Description: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
| Gene name | acoA |
| Synonyms | yfjK |
| Essential | no |
| Product | acetoin dehydrogenase E1 component (TPP-dependent alpha subunit) |
| Function | acetoin utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 35 kDa, 4.912 |
| Gene length, protein length | 999 bp, 333 aa |
| Immediate neighbours | yfjL, acoB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU08060
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (homogeneous) PubMed
Database entries
- Structure:
- UniProt: O31404
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
