Difference between revisions of "YxaL"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
Line 106: Line 106:
 
* '''Mutant:'''
 
* '''Mutant:'''
  
* '''Expression vector:''' pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
+
* '''Expression vector:'''  
       
+
** pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
* '''lacZ fusion:'''
+
** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP824, available in [[Stülke]] lab   
 +
   
 +
* '''lacZ fusion:''
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 16:56, 2 September 2009

  • Description: similar to Ser/Thr kinase

Gene name yxaL
Synonyms yxaK
Essential no
Product unknown
Function unknown
MW, pI 43 kDa, 6.379
Gene length, protein length 1230 bp, 410 aa
Immediate neighbours yxaM, yxaJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YxaL context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU39940

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP392 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP824, available in Stülke lab
  • 'lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References