Difference between revisions of "YxaL"
(→Biological materials) |
|||
Line 106: | Line 106: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
− | * '''Expression vector:''' pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | + | * '''Expression vector:''' |
− | + | ** pGP392 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | |
− | * '''lacZ fusion: | + | ** for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]]: pGP824, available in [[Stülke]] lab |
+ | |||
+ | * '''lacZ fusion:'' | ||
* '''GFP fusion:''' | * '''GFP fusion:''' |
Revision as of 16:56, 2 September 2009
- Description: similar to Ser/Thr kinase
Gene name | yxaL |
Synonyms | yxaK |
Essential | no |
Product | unknown |
Function | unknown |
MW, pI | 43 kDa, 6.379 |
Gene length, protein length | 1230 bp, 410 aa |
Immediate neighbours | yxaM, yxaJ |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU39940
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
Database entries
- Structure:
- UniProt: P42111
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- 'lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References