Difference between revisions of "SacT"
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| − | * '''Description:''' transcriptional antiterminator for the sacP-sacA operon <br/><br/> | + | * '''Description:''' transcriptional antiterminator for the ''[[sacP]]-[[sacA]]'' operon <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 102: | Line 102: | ||
=Biological materials = | =Biological materials = | ||
| − | * '''Mutant:''' | + | * '''Mutant:''' GP429 (spc), available in [[Stülke]] lab |
* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | + | ** for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP166, available in [[Stülke]] lab | |
| + | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP426, available in [[Stülke]] lab | ||
| + | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP427, available in [[Stülke]] lab | ||
| + | ** for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP439, available in [[Stülke]] lab | ||
| + | ** for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP440, available in [[Stülke]] lab | ||
| + | ** for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [[pGP570]]: pGP571, available in [[Stülke]] lab | ||
| + | ** for expression of the RNA-binding domain in ''B. subtilis'', in [[pBQ200]]: pGP446, available in [[Stülke]] lab | ||
| + | |||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
Revision as of 17:15, 2 September 2009
| Gene name | sacT |
| Synonyms | ipa-47d |
| Essential | no |
| Product | transcriptional antiterminator |
| Function | regulation of sucrose utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism, Stress | |
| MW, pI | 31 kDa, 5.587 |
| Gene length, protein length | 828 bp, 276 aa |
| Immediate neighbours | ywcJ, ywcI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU38070
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
- Protein family: transcriptional antiterminator bglG family (according to Swiss-Prot) BglG family of antiterminators
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P26212
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation: repressed by casamino acids PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP429 (spc), available in Stülke lab
- Expression vector:
- for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Stülke lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Stülke lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Stülke lab
- for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Stülke lab
- for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Stülke lab
- for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Stülke lab
- for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
