Difference between revisions of "RocA"
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | |||
* '''Operon:''' ''[[rocA]]-[[rocB]]-[[rocC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed] | * '''Operon:''' ''[[rocA]]-[[rocB]]-[[rocC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed] | ||
* '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed] | * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed] | ||
| − | * '''Regulation:''' induction by arginine ([[RocR] | + | * '''Regulation:''' |
| + | ** induction by arginine ([[RocR]], [[AhrC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162,7565595 PubMed] | ||
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| + | ** [[RocR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162 PubMed] | ||
| + | ** [[AhrC]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/7565595 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 122: | Line 124: | ||
=References= | =References= | ||
| − | <pubmed> | + | <pubmed>7565595 12618455,8113162 17218307, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:36, 3 February 2010
- Description: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
| Gene name | rocA |
| Synonyms | ipa-76d |
| Essential | no |
| Product | 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase |
| Function | arginine, ornithine and citrulline utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate | |
| MW, pI | 56 kDa, 5.58 |
| Gene length, protein length | 1545 bp, 515 aa |
| Immediate neighbours | rocB, rocG |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU37780
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H (according to Swiss-Prot)
- Protein family: RocA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P39634
- KEGG entry: [3]
- E.C. number: 1.5.1.12
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
