PnbA

• Description: para-nitrobenzyl esterase
  
  

Gene name	pnbA
Synonyms	estB
Essential	no
Product	para-nitrobenzyl esterase
Function	lipid degradation
MW, pI	53 kDa, 4.776
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	slrR, padC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34390

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Triacylglycerol + H₂O = diacylglycerol + a carboxylate (according to Swiss-Prot)

• Protein family: type-B carboxylesterase/lipase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-189 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1C7J

• UniProt: P37967

• KEGG entry: [2]

• E.C. number: 3.1.1.3 3.1.1.1]

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed during logarithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

J Zock, C Cantwell, J Swartling, R Hodges, T Pohl, K Sutton, P Rosteck, D McGilvray, S Queener
The Bacillus subtilis pnbA gene encoding p-nitrobenzyl esterase: cloning, sequence and high-level expression in Escherichia coli.
Gene: 1994, 151(1-2);37-43
[PubMed:7828905] [WorldCat.org] [DOI] (P p)