Difference between revisions of "DnaE"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[dnaE]]'' (according to [http://dbtbs.hgc.jp/COG/prom/dnaE.html DBTBS]) |
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** | + | ** induced by DNA damage ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed] | ** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed] | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 22:01, 18 December 2009
- Description: DNA polymerase III (alpha subunit)
| Gene name | dnaE |
| Synonyms | |
| Essential | yes PubMed |
| Product | DNA polymerase III (alpha subunit) |
| Function | DNA replication |
| MW, pI | 125 kDa, 6.02 |
| Gene length, protein length | 3345 bp, 1115 aa |
| Immediate neighbours | ytsJ, ytrI |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU29230
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
- Protein family: DnaE subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34623
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
Emmanuelle Le Chatelier, Olivier J Bécherel, Emmanuelle d'Alençon, Danielle Canceill, S Dusko Ehrlich, Robert P P Fuchs, Laurent Jannière
Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis.
J Biol Chem: 2004, 279(3);1757-67
[PubMed:14593098]
[WorldCat.org]
[DOI]
(P p)
E Dervyn, C Suski, R Daniel, C Bruand, J Chapuis, J Errington, L Jannière, S D Ehrlich
Two essential DNA polymerases at the bacterial replication fork.
Science: 2001, 294(5547);1716-9
[PubMed:11721055]
[WorldCat.org]
[DOI]
(P p)
