Difference between revisions of "GlcT"
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* '''Structure:''' | * '''Structure:''' | ||
− | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/O31691 O31691] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13880] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13880] |
Revision as of 07:50, 22 July 2009
- Description: Transcriptional antiterminator, controls expression of the ptsGHI operon
Gene name | glcT |
Synonyms | ykwA |
Essential | no |
Product | transcriptional antiterminator of the ptsGHI operon |
Function | transcriptional antiterminator |
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
MW, pI | 33,0 kDa, 7.01 |
Gene length, protein length | 855 bp, 285 amino acids |
Immediate neighbours | ykvZ, ptsG |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU13880
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcriptional antiterminator, RNA-binding protein, binds the ptsG RAT sequence
- Protein family: antiterminator of the BglG/ SacY family
Extended information on the protein
- Kinetic information:
- Domains:
- RNA-binding domain (N-terminal, constitutive antiterminator)
- 2x PTS regulation domains (PRDs) (C-terminal, neg. regulated by PtsG)
- Modification: phosphorylation (His104)
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: O31691
- KEGG entry: [2]
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP109 (in frame deletion), available in Stülke lab
- Expression vector: pGP124 (full length, in pWH844), pGP114 (amino acids 1-60, RNA-binding domain, in pWH844), pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in pWH844), pGP164 (both PRDs, in pWH844), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in pWH844), pGP424 (PRDI, in pWH844), pGP425 (PRDII, in pWH844), pGP442 (PRDI, in pGP570, with thrombin cleavage site), pGP443 (PRDII, in pGP570, with thrombin cleavage site), pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in pGP574), all available in Stülke lab
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References