Difference between revisions of "AsnO"
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[asnO]]'' | + | * '''Operon:''' ''[[asnO]]'' {{PubMed|10498721}} |
| − | * '''[[Sigma factor]]:''' [[ | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|10498721}} |
| − | * '''Regulation:''' expressed during sporulation ([[SigE]]) | + | * '''Regulation:''' |
| + | ** expressed during sporulation ([[SigE]]) {{PubMed|10498721}} | ||
| + | ** subject to positive regulation by [[SpoIIID]] {{PubMed|15383836}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| Line 120: | Line 122: | ||
=References= | =References= | ||
| − | <pubmed>10498721,, </pubmed> | + | <pubmed>10498721,15383836 , </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:48, 18 September 2009
- Description: asparagine synthase (glutamine-hydrolysing)
| Gene name | asnO |
| Synonyms | yisO, yucB |
| Essential | no |
| Product | asparagine synthase (glutamine-hydrolysing)) |
| Function | biosynthesis of asparagine |
| Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn | |
| MW, pI | 70 kDa, 5.696 |
| Gene length, protein length | 1842 bp, 614 aa |
| Immediate neighbours | yisN, yizA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU10790
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
- Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O05272
- KEGG entry: [3]
- E.C. number: 6.3.5.4
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721]
[WorldCat.org]
[DOI]
(P p)
